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Image Search Results
Journal: Genes to cells : devoted to molecular & cellular mechanisms
Article Title: Src family kinases suppress differentiation of brown adipocytes and browning of white adipocytes.
doi: 10.1111/gtc.12340
Figure Lengend Snippet: Figure 2 Effect of CSK-over-expression on brown adipogenesis. (A–C) Wild-type CSK (CSKWT) and kinase-deficient CSK (CSKK222R) were lentivirally infected to brown preadipocytes 2 days before induction of differentiation. (A) Immunoblotting for CSK (~52 kDa), P-Tyr527-SFK (~55 kDa), and P-Tyr416-SFK (~55 kDa). a-Tubulin (~50 kDa) was blotted as a loading control. (B) Immunostaining for Ucp1 (red). Nuclei were shown by Hoechst staining (blue), and lipid droplets formation was assessed with BODIPY (green). Percentages of Ucp1-positive cells. Scale bars, 100 lm. Values are the means SE (n = 3) *P ≤0.05 vs con- trol, assessed by Student’s t-test. (C) mRNA levels of Ucp1 at days 0, 4, and 7.
Article Snippet: Antibodies were purchased as follows: rabbit anti-Ucp1 (ab10983) antibodies from Abcam; rabbit anti-phospho-Src family (Tyr416) (D49G4) (#6943) and
Techniques: Over Expression, Infection, Western Blot, Control, Immunostaining, Staining