rabbit antibody non phospho src tyr527 Search Results


96
Cell Signaling Technology Inc rabbit anti phospho src tyr527 2105 antibodies
Figure 2 Effect of CSK-over-expression on brown adipogenesis. (A–C) Wild-type CSK (CSKWT) and kinase-deficient CSK (CSKK222R) were lentivirally infected to brown preadipocytes 2 days before induction of differentiation. (A) Immunoblotting for CSK (~52 kDa), <t>P-Tyr527-SFK</t> (~55 kDa), and P-Tyr416-SFK (~55 kDa). a-Tubulin (~50 kDa) was blotted as a loading control. (B) Immunostaining for Ucp1 (red). Nuclei were shown by Hoechst staining (blue), and lipid droplets formation was assessed with BODIPY (green). Percentages of Ucp1-positive cells. Scale bars, 100 lm. Values are the means SE (n = 3) *P ≤0.05 vs con- trol, assessed by Student’s t-test. (C) mRNA levels of Ucp1 at days 0, 4, and 7.
Rabbit Anti Phospho Src Tyr527 2105 Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Signaling Technology Inc rabbit antibody non phospho src tyr527
Figure 2 Effect of CSK-over-expression on brown adipogenesis. (A–C) Wild-type CSK (CSKWT) and kinase-deficient CSK (CSKK222R) were lentivirally infected to brown preadipocytes 2 days before induction of differentiation. (A) Immunoblotting for CSK (~52 kDa), <t>P-Tyr527-SFK</t> (~55 kDa), and P-Tyr416-SFK (~55 kDa). a-Tubulin (~50 kDa) was blotted as a loading control. (B) Immunostaining for Ucp1 (red). Nuclei were shown by Hoechst staining (blue), and lipid droplets formation was assessed with BODIPY (green). Percentages of Ucp1-positive cells. Scale bars, 100 lm. Values are the means SE (n = 3) *P ≤0.05 vs con- trol, assessed by Student’s t-test. (C) mRNA levels of Ucp1 at days 0, 4, and 7.
Rabbit Antibody Non Phospho Src Tyr527, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Signaling Technology Inc human p src
Figure 2 Effect of CSK-over-expression on brown adipogenesis. (A–C) Wild-type CSK (CSKWT) and kinase-deficient CSK (CSKK222R) were lentivirally infected to brown preadipocytes 2 days before induction of differentiation. (A) Immunoblotting for CSK (~52 kDa), <t>P-Tyr527-SFK</t> (~55 kDa), and P-Tyr416-SFK (~55 kDa). a-Tubulin (~50 kDa) was blotted as a loading control. (B) Immunostaining for Ucp1 (red). Nuclei were shown by Hoechst staining (blue), and lipid droplets formation was assessed with BODIPY (green). Percentages of Ucp1-positive cells. Scale bars, 100 lm. Values are the means SE (n = 3) *P ≤0.05 vs con- trol, assessed by Student’s t-test. (C) mRNA levels of Ucp1 at days 0, 4, and 7.
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Cell Signaling Technology Inc rabbit monoclonal anti phospho src
Figure 2 Effect of CSK-over-expression on brown adipogenesis. (A–C) Wild-type CSK (CSKWT) and kinase-deficient CSK (CSKK222R) were lentivirally infected to brown preadipocytes 2 days before induction of differentiation. (A) Immunoblotting for CSK (~52 kDa), <t>P-Tyr527-SFK</t> (~55 kDa), and P-Tyr416-SFK (~55 kDa). a-Tubulin (~50 kDa) was blotted as a loading control. (B) Immunostaining for Ucp1 (red). Nuclei were shown by Hoechst staining (blue), and lipid droplets formation was assessed with BODIPY (green). Percentages of Ucp1-positive cells. Scale bars, 100 lm. Values are the means SE (n = 3) *P ≤0.05 vs con- trol, assessed by Student’s t-test. (C) mRNA levels of Ucp1 at days 0, 4, and 7.
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Cell Signaling Technology Inc active fyn
Figure 2 Effect of CSK-over-expression on brown adipogenesis. (A–C) Wild-type CSK (CSKWT) and kinase-deficient CSK (CSKK222R) were lentivirally infected to brown preadipocytes 2 days before induction of differentiation. (A) Immunoblotting for CSK (~52 kDa), <t>P-Tyr527-SFK</t> (~55 kDa), and P-Tyr416-SFK (~55 kDa). a-Tubulin (~50 kDa) was blotted as a loading control. (B) Immunostaining for Ucp1 (red). Nuclei were shown by Hoechst staining (blue), and lipid droplets formation was assessed with BODIPY (green). Percentages of Ucp1-positive cells. Scale bars, 100 lm. Values are the means SE (n = 3) *P ≤0.05 vs con- trol, assessed by Student’s t-test. (C) mRNA levels of Ucp1 at days 0, 4, and 7.
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93
Cell Signaling Technology Inc src antibody sampler kit
Figure 2 Effect of CSK-over-expression on brown adipogenesis. (A–C) Wild-type CSK (CSKWT) and kinase-deficient CSK (CSKK222R) were lentivirally infected to brown preadipocytes 2 days before induction of differentiation. (A) Immunoblotting for CSK (~52 kDa), <t>P-Tyr527-SFK</t> (~55 kDa), and P-Tyr416-SFK (~55 kDa). a-Tubulin (~50 kDa) was blotted as a loading control. (B) Immunostaining for Ucp1 (red). Nuclei were shown by Hoechst staining (blue), and lipid droplets formation was assessed with BODIPY (green). Percentages of Ucp1-positive cells. Scale bars, 100 lm. Values are the means SE (n = 3) *P ≤0.05 vs con- trol, assessed by Student’s t-test. (C) mRNA levels of Ucp1 at days 0, 4, and 7.
Src Antibody Sampler Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
Cell Signaling Technology Inc rabbit anti phospho src tyr527
Figure 2 Effect of CSK-over-expression on brown adipogenesis. (A–C) Wild-type CSK (CSKWT) and kinase-deficient CSK (CSKK222R) were lentivirally infected to brown preadipocytes 2 days before induction of differentiation. (A) Immunoblotting for CSK (~52 kDa), <t>P-Tyr527-SFK</t> (~55 kDa), and P-Tyr416-SFK (~55 kDa). a-Tubulin (~50 kDa) was blotted as a loading control. (B) Immunostaining for Ucp1 (red). Nuclei were shown by Hoechst staining (blue), and lipid droplets formation was assessed with BODIPY (green). Percentages of Ucp1-positive cells. Scale bars, 100 lm. Values are the means SE (n = 3) *P ≤0.05 vs con- trol, assessed by Student’s t-test. (C) mRNA levels of Ucp1 at days 0, 4, and 7.
Rabbit Anti Phospho Src Tyr527, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Signaling Technology Inc rabbit monoclonal anti phosphorylated
Figure 2 Effect of CSK-over-expression on brown adipogenesis. (A–C) Wild-type CSK (CSKWT) and kinase-deficient CSK (CSKK222R) were lentivirally infected to brown preadipocytes 2 days before induction of differentiation. (A) Immunoblotting for CSK (~52 kDa), <t>P-Tyr527-SFK</t> (~55 kDa), and P-Tyr416-SFK (~55 kDa). a-Tubulin (~50 kDa) was blotted as a loading control. (B) Immunostaining for Ucp1 (red). Nuclei were shown by Hoechst staining (blue), and lipid droplets formation was assessed with BODIPY (green). Percentages of Ucp1-positive cells. Scale bars, 100 lm. Values are the means SE (n = 3) *P ≤0.05 vs con- trol, assessed by Student’s t-test. (C) mRNA levels of Ucp1 at days 0, 4, and 7.
Rabbit Monoclonal Anti Phosphorylated, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
GeneTex anti-c-src (phospho tyr 527) antibody
Figure 2 Effect of CSK-over-expression on brown adipogenesis. (A–C) Wild-type CSK (CSKWT) and kinase-deficient CSK (CSKK222R) were lentivirally infected to brown preadipocytes 2 days before induction of differentiation. (A) Immunoblotting for CSK (~52 kDa), <t>P-Tyr527-SFK</t> (~55 kDa), and P-Tyr416-SFK (~55 kDa). a-Tubulin (~50 kDa) was blotted as a loading control. (B) Immunostaining for Ucp1 (red). Nuclei were shown by Hoechst staining (blue), and lipid droplets formation was assessed with BODIPY (green). Percentages of Ucp1-positive cells. Scale bars, 100 lm. Values are the means SE (n = 3) *P ≤0.05 vs con- trol, assessed by Student’s t-test. (C) mRNA levels of Ucp1 at days 0, 4, and 7.
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99
Cell Signaling Technology Inc goat anti rabbit igg
Figure 2 Effect of CSK-over-expression on brown adipogenesis. (A–C) Wild-type CSK (CSKWT) and kinase-deficient CSK (CSKK222R) were lentivirally infected to brown preadipocytes 2 days before induction of differentiation. (A) Immunoblotting for CSK (~52 kDa), <t>P-Tyr527-SFK</t> (~55 kDa), and P-Tyr416-SFK (~55 kDa). a-Tubulin (~50 kDa) was blotted as a loading control. (B) Immunostaining for Ucp1 (red). Nuclei were shown by Hoechst staining (blue), and lipid droplets formation was assessed with BODIPY (green). Percentages of Ucp1-positive cells. Scale bars, 100 lm. Values are the means SE (n = 3) *P ≤0.05 vs con- trol, assessed by Student’s t-test. (C) mRNA levels of Ucp1 at days 0, 4, and 7.
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92
Biorbyt phospho src tyr527 orb14869 antibody
Figure 2 Effect of CSK-over-expression on brown adipogenesis. (A–C) Wild-type CSK (CSKWT) and kinase-deficient CSK (CSKK222R) were lentivirally infected to brown preadipocytes 2 days before induction of differentiation. (A) Immunoblotting for CSK (~52 kDa), <t>P-Tyr527-SFK</t> (~55 kDa), and P-Tyr416-SFK (~55 kDa). a-Tubulin (~50 kDa) was blotted as a loading control. (B) Immunostaining for Ucp1 (red). Nuclei were shown by Hoechst staining (blue), and lipid droplets formation was assessed with BODIPY (green). Percentages of Ucp1-positive cells. Scale bars, 100 lm. Values are the means SE (n = 3) *P ≤0.05 vs con- trol, assessed by Student’s t-test. (C) mRNA levels of Ucp1 at days 0, 4, and 7.
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Image Search Results


Figure 2 Effect of CSK-over-expression on brown adipogenesis. (A–C) Wild-type CSK (CSKWT) and kinase-deficient CSK (CSKK222R) were lentivirally infected to brown preadipocytes 2 days before induction of differentiation. (A) Immunoblotting for CSK (~52 kDa), P-Tyr527-SFK (~55 kDa), and P-Tyr416-SFK (~55 kDa). a-Tubulin (~50 kDa) was blotted as a loading control. (B) Immunostaining for Ucp1 (red). Nuclei were shown by Hoechst staining (blue), and lipid droplets formation was assessed with BODIPY (green). Percentages of Ucp1-positive cells. Scale bars, 100 lm. Values are the means SE (n = 3) *P ≤0.05 vs con- trol, assessed by Student’s t-test. (C) mRNA levels of Ucp1 at days 0, 4, and 7.

Journal: Genes to cells : devoted to molecular & cellular mechanisms

Article Title: Src family kinases suppress differentiation of brown adipocytes and browning of white adipocytes.

doi: 10.1111/gtc.12340

Figure Lengend Snippet: Figure 2 Effect of CSK-over-expression on brown adipogenesis. (A–C) Wild-type CSK (CSKWT) and kinase-deficient CSK (CSKK222R) were lentivirally infected to brown preadipocytes 2 days before induction of differentiation. (A) Immunoblotting for CSK (~52 kDa), P-Tyr527-SFK (~55 kDa), and P-Tyr416-SFK (~55 kDa). a-Tubulin (~50 kDa) was blotted as a loading control. (B) Immunostaining for Ucp1 (red). Nuclei were shown by Hoechst staining (blue), and lipid droplets formation was assessed with BODIPY (green). Percentages of Ucp1-positive cells. Scale bars, 100 lm. Values are the means SE (n = 3) *P ≤0.05 vs con- trol, assessed by Student’s t-test. (C) mRNA levels of Ucp1 at days 0, 4, and 7.

Article Snippet: Antibodies were purchased as follows: rabbit anti-Ucp1 (ab10983) antibodies from Abcam; rabbit anti-phospho-Src family (Tyr416) (D49G4) (#6943) and rabbit anti-phospho-Src (Tyr527) (#2105) antibodies from Cell Signaling; rabbit anti-CSK (C20) antibodies from Santa Cruz; mouse anti-a-tubulin (DM1A) antibody from Sigma; Alexa Fluor 594-goat anti-rabbit IgG (#A11037) from Molecular Probes; sheep anti-mouse IgG horseradish peroxidase (HRP)-conjugated (NA9310) and donkey anti-rabbit IgG HRP-conjugated (NA9340) from GE Healthcare.

Techniques: Over Expression, Infection, Western Blot, Control, Immunostaining, Staining